Why choose rabbit monoclonal antibodies for your antibody development process? Rabbits offer a strategic advantage to mice in terms of monoclonal antibody production. The rabbit spleen contains more B cells than the mouse spleen because rabbits are larger.
The more B cells, the more antibodies are produced, which means a greater chance of finding the ideal clone to thrive. There are many companies available that provide the best rabbit monoclonal antibody discovery service.
Antibodies to a wider range of epitopes
The rabbit immunoglobulin gene produces antibodies that respond to a much wider range of epitopes than mouse antibodies. Protein isoforms can differ by one amino acid residue, leading to slight structural aberrations.
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The rabbit's immune system recognizes these subtle differences better by producing monoclonal antibodies. On average, rabbit monoclonal antibodies bind with 10-100-fold higher affinity than mouse monoclonal antibodies.
More small molecules are immunogenic in rabbits than in mice
The rabbit immune system is able to generate immunogenic responses to small molecules much more easily than the mouse immune system. When a small molecule with a small epitope was injected into rabbits and mice side by side, rabbits were more likely to have high titers than mice because rabbits had more complex immune responses.
Immune dominance occurs when certain epitopes of the same antigen are more immunogenic than others. As a result, the immune system produces a large number of antibodies against the dominant immunogenic epitope and few antibodies against other epitopes. Rabbits showed lower immunodominance than mice.